Automated estimation of urinary calcium using the Eppendorf flame photometer

Automated estimation of urinary calcium using the Eppendorf flame photometer.

A method is described for estimating urine calcium on a mechanized Eppendorf flame photometer with twin channels at a rate of 60 per hour. Sodium is estimated simultaneously so that a correction can be made for the small sodium error. Errors due to potassium and phosphate are minimized by adding these ions to the standards and to the diluting fluid. Results agree well with those obtained by Tri...

Automated Estimation of Sodium, Potassium, and Calcium Using the Eppendorf Flame Photometer.

An Eppendorf flame photometer may be used to estimate sodium, potassium, and calcium in plasma which has been automatically sampled and diluted. Readings may be automatically recorded by feeding the output from the photomultiplier into a sensitive recorder. This method estimates 60 samples an hour, using approximately 0.12 ml. plasma per estimation. The accuracy of the method is discussed.

Serum Calcium Using the Eel Flame Photometer

Flame photometry has many applications, and in the clinical field its use in the estimation of serum sodium and potassium is now well established. Difficulties have been encountered in the estimation of serum calcium by flame photometry, since the emission spectrum of calcium is relatively weak and is subject to interference by the presence of other ions, e.g., sodium. Although many methods hav...

Microdetermination of serum calcium using the Eel flame photometer.

The quantitative estimation of amino nitrogen by determination of bound copper with the flame photometer.

The measurement of combined copper has been used in a number of procedures for the quantitative determination of amino acids and peptides. Pope and Stevens (1) as well as Schroeder, Kay, and Mills (2) determined the combined copper iodometrically. Martin and Mittelmann (3) analyzed for copper with the polarograph. Woiwod (4) used the spectrophotometer to measure the color produced by the reacti...